Cytometry allows the measurement of optical properties and fluorescence of large numbers of cells or particles at high speed. The main advantage of flow cytometry is the speed of acquisition of data, while collecting fluorescent parameters on large numbers of cells, therefore allowing the analysis of complex or rare subpopulations.
Flow cytometry is routinely used to quantify multiple cellular markers or functions such as cell viability, proliferation, cell cycle, cytokine secretion, protein phosphorylation, microparticles quantification… When associated with a cell sorting system, populations of interest can be isolated for cell culture or further molecular analysis.
The plateform is also equipped with an Image Cytometer, able to capture high-resolution images of shape and fluorescence from cells in flow at rates of up to 1,000 cells per second. Combination of flow cytometry high-throughput power with image resolution allows acquisition of statistically robust data on the detailed structure of cells and the location of intracellular components, and the study of endocytosis, phagocytosis, cell-cell interaction, colocalization or in situ hybridization.
Camille Knosp, PhD, IE UPC
Facility Manager
Corinne Tanchot
Scientific advisor
LSR Fortessa analyzer
3 Lasers 407nm, 488nm and 633nm, 11 colors, 2 scatters and FSC-PMT
Aria II SORP Cell Sorter
4 Lasers 355nm, 405nm, 488nm and 640 nm, 13 colors and 2 scatters
Collection into tubes (1.5 to 15 mL), plates (6 to 384 wells) or onto slides
CYTOFLEX ANALYZER
3 Lasers 405nm, 488nm and 638nm, 13 colors, 3 scatters
LSRFortessa X-20
5 lasers: 375 nm, 405nm, 488nm, 561nm, and 640nm, 18 colors and 2 scatters
FLOW IMAGER IMAGESTREAM MKII
Lasers 350nm, 488nm, 561nm and 633nm, 12 channels and 2 scatters, 20x, 40x, 60x objectives
Training: The platform trains new users until they become independent to use the instruments and conduct their experiments. Our goal is to ensure the robustness of the data generated and to provide continuous training for the users of the platform.
Every year, the platform organizes :
Theorical trainings : several class of 1H are offer each year addressed to beginners and more experienced users
Practical training : 3h in small groups
FlowJo training : 1 full day training with the the FlowJO application specialist
Consulting:
We offer one on one assistance for every new cytometry project
We accompany researchers with panel design
We provide advice on experimental procedures and data analysis
2013 CORDIM – 400 K€
2009 LEDUCQ Fundation – 500 K€