Cytometry enables the rapid, high-throughput measurement of the optical properties and fluorescence of large numbers of cells or particles. The primary advantage of flow cytometry lies in its speed of data acquisition, allowing for the collection of fluorescent parameters from vast cell populations. This capability is crucial for analyzing complex or rare subpopulations within a sample. Flow cytometry is routinely employed to quantify multiple cellular markers or functions, including cell viability, proliferation, cell cycle, cytokine secretion, protein phosphorylation, and microparticle quantification. When combined with a cell sorting system, specific populations of interest can be isolated for subsequent cell culture or further molecular analysis.
FLOW CYTOMETRY Facility Manager
Our platform is equipped with an Image Cytometer, capable of capturing high-resolution images of cell shape and fluorescence in flow at speeds up to 1,000 cells per second. This combination of flow cytometry’s high-throughput power with imaging resolution allows for the acquisition of statistically robust data on the detailed structure of cells and the precise localization of intracellular components. It further enables advanced studies such as endocytosis, phagocytosis, cell-cell interaction, colocalization, and in situ hybridization.
Our Instrumentation Includes:
· LSR Fortessa Analyzer : 3 Lasers: 407nm, 488nm, and 633nm ; 11 Colors, 2 scatters, and FSC-PMT
· Aria II SORP Cell Sorter: 4 Lasers: 355nm, 405nm, 488nm, and 640nm ; 13 Colors and 2 scatters ; Collection into tubes (1.5 to 15 mL), plates (6 to 384 wells), or onto slides
· Cytoflex Analyser : 3 Lasers: 405nm, 488nm, and 638nm ; 13 Colors, 3 scatters
. LSRFortessa X-20: 5 Lasers: 375 nm, 405nm, 488nm, 561nm, and 640nm ; 18 Colors and 2 scatters
· Flow Imager Imagestream MKII: Lasers: 350nm, 488nm, 561nm, and 633nm ; 12 Channels and 2 scatters ; 20x, 40x, 60x objectives
Head of Platform: Camille Knosp Email: camille.knosp@inserm.fr
Training: The platform offers comprehensive training for new users, guiding them until they achieve independence in operating the instruments and conducting their experiments. Our goal is to ensure the robustness of generated data and to provide continuous education for all platform users. Each year, the platform organizes:
· Theoretical Trainings: Several 1-hour classes offered annually, suitable for both beginners and experienced users.
· Practical Trainings: 3-hour sessions conducted in small groups.
· FlowJo Training: A full-day training session with a FlowJo application specialist.
Consulting: We provide personalized support through:
· One-on-one assistance for every new cytometry project.
· Guidance for researchers with panel design.
· Expert advice on experimental procedures and data analysis.
