Cytometry allows the measurement of optical properties and fluorescence of large numbers of cells or particles at high speed. The main advantage of flow cytometry is the speed of acquisition of data, while collecting fluorescent parameters on large numbers of cells, therefore allowing the analysis of complex or rare subpopulations.
Flow cytometry is routinely used to quantify multiple cellular markers or functions such as cell viability, proliferation, cell cycle, cytokine secretion, protein phosphorylation, microparticles quantification… When associated with a cell sorting system, populations of interest can be isolated for cell culture or further molecular analysis.
The plateform is also equipped with an Image Cytometer, able to capture high-resolution images of shape and fluorescence from cells in flow at rates of up to 1,000 cells per second. Combination of flow cytometry high-throughput power with image resolution allows acquisition of statistically robust data on the detailed structure of cells and the location of intracellular components, and the study of endocytosis, phagocytosis, cell-cell interaction, colocalization or in situ hybridization.
- Camille Knosp: Facility Manager
- Corinne Tanchot: Scientific advisor